Effect of acupuncture at "Zhibian" (BL 54) through "Shuidao" (ST 28) on the expression of apoptosis-related factors in rats with premature ovarian insufficiency based on oxidative stress / 中国针灸

OBJECTIVE@#To explore the possible mechanism of acupuncture at "Zhibian" (BL 54) through "Shuidao" (ST 28) on premature ovarian insufficiency (POI) from the perspective of oxidative stress.@*METHODS@#Sixty female SD rats were randomly divided into a blank group, a model group, a sham acupuncture group, a medication group, and an acupuncture group, 12 rats in each group. Except the blank group, the rats in the remaining groups were intraperitoneally injected with cyclophosphamide to establish the POI model. After the model was successfully established, the rats in the acupuncture group were treated with acupuncture at "Zhibian" (BL 54) through "Shuidao" (ST 28), with a depth of about 12 mm, and the needle was retained for 30 min; the acupuncture was given once a day, for a total of 4 weeks. The rats in the sham acupuncture group were treated with blunt-head needle to tap the skin surface of "Zhibian" (BL 54), without penetrating the skin, once a day for 4 weeks. The rats in the medication group were treated with estradiol valerate by gastric gavage for 4 weeks. After the intervention, the level of reactive oxygen species (ROS) in the ovarian tissue was detected by fluorescence probe; the expression of c-Jun N-terminal kinase (JNK), forkhead box O1 (FoxO1), tumor suppressor gene protein 53 (p53) and p53 up-regulated modulator of apoptosis (Puma) mRNA and protein in ovarian tissue were detected by real-time fluorescence quantitative PCR and Western blot.@*RESULTS@#Compared with the blank group, the level of ROS and the expression of JNK mRNA, p-JNK protein, FoxO1, p53, Puma mRNA and protein in the ovarian tissue in the model group were increased (P<0.01). Compared with the model group, the level of ROS and the expression of p-JNK protein, FoxO1, p53, Puma mRNA and protein in the ovarian tissue in the sham acupuncture group were slightly reduced, but the difference was not statistically significant (P>0.05). The level of ROS and the expression of JNK mRNA, p-JNK protein, FoxO1, p53, Puma mRNA and protein in the ovarian tissue in the acupuncture group and the medication group were reduced (P<0.01).@*CONCLUSION@#Acupuncture at "Zhibian" (BL 54) through "Shuidao" (ST 28) could improve the level of oxidative stress, down-regulate the expression of apoptosis-related factors JNK, FoxO1, p53 and Puma induced by oxidative stress, and inhibit the premature failure of ovarian reserve function caused by apoptosis of ovarian granulosa cells in POI rats.

The relaxation of zonule in primary angle closure suspects / 眼科新进展

Objecttve To compare the difference of relaxation distribution of zonule in different orientations between primary angle closure suspects (PACS) and normal controls,and to explore the role of latent subluxated lens in the pathogenesis of glaucoma.Methods Together 19 eyes of 19 PACS (PACS group) and 19 eyes of 19 age-,gender-and eye-matched normal controls (control group) were enrolled in this study.Pentacam was performed under standardized dark conditions before and 30 min after instillation of compound tropicamide eye drop.Peripheral anterior chamber depth (peri-ACD) of 8 orientations (superior,temporal superior,temporal,temporal inferior,inferior,nasal inferior,nasal,nasal superior position) in the central 4 mm diameter was measured automatically in each eye.The differences of peri-ACD (△ peri-ACD) before and after mydriasis were measured to reflex the relaxation of zonule.The difference of peri-ACD in 8 orientations after mydriasis could confirm the possibility of latent subluxated lens,and its range could verify the degree of lens deviation.Results Significant difference was observed in each groups in terms of △peri-ACD (P＜0.001,0.043).The △peri-ACD of the temporal inferior position was (0.40 ± 0.28)mm in PACS group,which was smaller than that in the control group [(0.55 ±0.15)mm] (P =0.041),but no significant difference was observed between PACS patients and normal controls in other orientations (all P ＞ 0.05).And significant difference was detected between the 8 positions of peri-ACD after mydriasis in each groups (P =0.001,0.009),and these parameters between the two groups were also significantly different (all P ＜ 0.01).Moreover,there was no significant difference in the difference of between the minimum and maximum peri-ACD in the PACS group and the control group [(0.35 ± 0.18)mm vs.(0.43 ± 0.28) mm] (P =0.362).Conclusion The uneven relaxation distribution of zonule may exist in both PACS patients and normal controls,and the relaxation of zonule may be significant in temporal inferior position in PACS patients.

Influence of Montelukast on Plasma Nitric Oxide in Preschool Children with Asthma / 实用儿科临床杂志

Objective To explore the influence of montelukast on plasma nitric oxide in preschool children with asthma.Methods Forty-four preschool children with asthma aged 2-5 years who firstly met a criterion of asthma and treated 4 weeks with montelukast were investigated;and nitric oxide levels of plasma were inspected respectively before treatment and after treatment 1 week,4 weeks.Results The level of nitric oxide in the plasma of asthmatic children was obviously higher than that in normal control group(P